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I'm installing Ubuntu 16.04 (64bit) using a Pen drive but during the installation, I get the I/O error, however sometimes prior to this, some other errors appear saying I am missing a certain file and then I have the option to Cancel, Retry or Skip (the same error doesn't always appear every time during the installation) I'm on a HP Compaq dx2300 microtower. I have tried looking at other solutions, but they do not seem to work for me.

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After 2 days of trying, I found a solution for my problems (similar to yours). I tested on different OSes running on different computers (win7 32-bit & win8.1 64-bit), different USB, different Ubuntu version (14.04/16.04 32-bit/64-bit). All of them return the same error at the same process. Although checking disk before installing warns that splashfs file has an error, errno 5 is not from there.

Finally, I found a solution: Set partitions for the USB you want to install Ubuntu onto as shown.

The problem could be from the first FAT32 partition for boot loader. The size of that partition should be lower than 256MB. If you set bootloader in another disk, this partition could be ignored.

The free space is used to create a normal partition which stores data after finishing installation of Ubuntu.