I have looked through similar examples but struggling to get this to work with my data. I want to split one column of data into two, Ortho Group and Score.

I have looked through similar examples but struggling to get this to work with my data. I want to split one column of data into two, Ortho Group and Score.

Assuming df is the name of your data frame, you can try tstrsplit from data.table, e.g.,
library(data.table)
setDT(df)[,tstrsplit(Orthogroup.Mean_Identity,",")]
Using separate from the tidyr package and assuming your data is called df:
separate(df, col = Orthogroup.Mean_Identity, sep = ",", into = c("Orthogroup", "Mean_Identity"))