There are 3 solutions (and probably 100 more):
The"classic" for method appending everyone of the N=4 letters to the same string and the choice method of the random module that picks one element among its arguments,
Using the join method to add in one time the elements generated by the choices method of the random module. The choices method is very versatile and can be used with several arguments (official documentation),
Using the join method to add in one time the elements generated by the sample method of the random module. The sample(set,num)method picks randomly num elements in set.
import random
N=4
source_string = 'ABCDEF
Method1
random_string_1=""
for it in range(0,N):
random_string_1 = random_string_1 + random.choice(source_string)
Method2
random_string_2 = "".join(random.choices(source_string, weights=None,
cum_weights=None, k=N))
Method3
random_string_3 = "".join(random.sample(source_string, N))